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The antiviral activity of compounds isolated from Kenyan
Carissa edulis (Forssk.) Vahl
F. M. Tolo et al.
F. M. Tolo et al.
(Journal of Medicinal Plants Research Vol. 4(15), pp. 1517-1522, 4 August, 2010)
An Abstract
Extracts of a Kenyan medicinal plant, Carissa edulis (Forssk.) Vahl, were subjected to bioactivity guided phytochemical analysis for identification of biological makers of activity. Pure compounds; lupeol, oleuropein, carissol and -amyrin were subsequently isolated. In vitro evaluations of the compounds against viral strains of Herpes simples virus types 1 revealed a significant activity for lupeol at EC50 of between 2.98 - 4.2 μg/ml for both sensitive and resistant strains with a high therapeutic index (TI > 38).
A concentration of the compound at 10.0 μg/ml was virucidal, reducing viral yields in Vero E6 cells by 98.3%. On oral administration to mice at 20.0 μg/ml following a cutaneous viral infection, a delayed onset of infections of slow progression to mild and sever zosteriform lesions were observed (p £ 0.05 test vs. control by repeated measures ANOVA).
The mice receiving treatment with the compound also exhibited increased mean survival times as opposed to control (p 0.05 test verses control by Student's t-test) with a therapeutic index >5 (LD50 >100.0 μg/ml). The results indicate promising antiviral
activity of lupeol and necessitate further examinations of efficacy and safety in higher mammals.
A concentration of the compound at 10.0 μg/ml was virucidal, reducing viral yields in Vero E6 cells by 98.3%. On oral administration to mice at 20.0 μg/ml following a cutaneous viral infection, a delayed onset of infections of slow progression to mild and sever zosteriform lesions were observed (p £ 0.05 test vs. control by repeated measures ANOVA).
The mice receiving treatment with the compound also exhibited increased mean survival times as opposed to control (p 0.05 test verses control by Student's t-test) with a therapeutic index >5 (LD50 >100.0 μg/ml). The results indicate promising antiviral
activity of lupeol and necessitate further examinations of efficacy and safety in higher mammals.
To access full paper visit, Journal of Medicinal Plants Research